Course image Biochemistry - Nucleic Acids and Buffer Systems
M Sc Applied Chemistry

1.Nucleic acids:Nucleacid bases, Nucleosides, nucleotides, structure of DNA, RNA and its classifications, Replication of DNA, transcription, transalation and Protein Biosynthesis. DNA finger printing Techniques, Introduction to Recombinant DNA technology. Genetic code, gene therapy (basic concept only), PCR. Chemical Synthesis of Nucleotides, Restriction enzymes. Chemistry of ATP,ADP and AMP.

Buffer in pharmaceutical and biological system, pH, the buffer equation (Henderson Hesselbach), Buffer calculations, three important buffer systems in human body, buffer capacity, osmotic pressure and tonicity, pharmaceutical buffers, preparations of pharmaceutical buffer solutions.